Alternative Routes for the Synthesis of 5-Aminolevulinic Acid in Maize Leaves

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Alternative Routes for the Synthesis of 5-Aminolevulinic Acid in Maize Leaves : II. Formation from Glutamate.

Intact plastids from greening maize (Zea mays L.) leaves converted [(14)C]glutamate and [(14)C]2-ketoglutarate (KG) to [(14)C]5-aminolevulinic acid (ALA). Glutamate appeared to be the immediate precursor of ALA, while KG was first converted to glutamate, as shown by the effect of various inhibitors of amino acid metabolism. Plastids from greening leaves contained markedly higher activity as com...

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Alternative Routes for the Synthesis of 5-Aminolevulinic Acid in Maize Leaves : I. Formation from 2-Ketoglutarate via 4,5-Dioxovaleric Acid.

Cell-free extracts from greening maize (Zea mays L.) leaves catalyze the conversion of [(14)C]2-ketoglutarate (KG) to [(14)C]5-aminolevulinic acid (ALA) in a reaction which requires NADH and an amino donor and shows maximal activity around pH 6.5. The enzymic system is located in the cytosol. This cell fraction contains a low level of ;KG dehydrogenase' activity and a transaminase which catalyz...

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ژورنال

عنوان ژورنال: Plant Physiology

سال: 1983

ISSN: 0032-0889,1532-2548

DOI: 10.1104/pp.72.4.1062